For links to paper pdf files, and additional publications, click here

Selected publications:

Frampton, S. L., Sutcliffe, C., Baldock, C. and Ashe, H. L. (2022) ‘Modelling the structure of Short Gastrulation and generation of a toolkit for studying its function in Drosophila’, Biology Open 11(6)
PMID: 35603711

Bowles, J. R., Hoppe, C., Ashe, H. L. and Rattray, M. (2021) ‘Scalable inference of transcriptional kinetic parameters from MS2 time series data’, Bioinformatics 38(4):1030-6
PMID: 34788793

Vinter, D. J., Hoppe, C. and Ashe, H. L. (2021) ‘Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo’, STAR Protocols 2(3): 100812
PMID: 34585149

Vinter, D. J., Hoppe, C., Minchington, T. G., Sutcliffe, C. and Ashe, H. L. (2021) ‘Dynamics of hunchback translation in real time and at single mRNA resolution in the Drosophila embryo’, Development 148(18): dev196121
PMID: 33722899

Hoppe, C. and Ashe, H. L. (2021) ‘Live imaging and quantitation of nascent transcription using the MS2/MCP system in the Drosophila embryo’, STAR Protocols 2(1): 100379
PMID: 33778778

Hoppe, C. and Ashe, H. L. (2021) ‘CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos’, STAR Protocols 2(1): 100379
PMID: 33786461

Wilcockson, S. G. and Ashe, H. L. (2021) ‘Live imaging of the Drosophila ovarian germline stem cell niche’, STAR Protocols 2(1): 100371
PMID: 33733240

Hoppe, C., Bowles, J. R., Minchington, T. G., Sutcliffe, C., Upadhyai, P., Rattray, M. and Ashe, H. L. (2020) ‘Modulation of the Promoter Activation Rate Dictates the Transcriptional Response to Graded BMP Signaling Levels in the Drosophila Embryo’, Developmental Cell 54(6): 727–741.
PMID: 32758422

Wilcockson, S. G. and Ashe, H. L. (2019) ‘Drosophila Ovarian Germline Stem Cell Cytocensor Projections Dynamically Receive and Attenuate BMP Signaling’, Developmental Cell 50(3): 296–312.
PMID: 31178401

Wilcockson, S. G., Sutcliffe, C. and Ashe, H. L. (2017) ‘Control of signaling molecule range during developmental patterning’, Cell Mol Life Sci 74(11): 1937-1956.
PMID: 27999899

Ashe, H. L. (2016) ‘Modulation of BMP signalling by integrins’, Biochemical Society Transactions 44(5): 1465-1473.
PMID: 27911728

Deignan, L., Pinheiro, M. T., Sutcliffe, C., Saunders, A., Wilcockson, S. G., Zeef, L. A., Donaldson, I. J. and Ashe, H. L. (2016) ‘Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo’, PLoS Genetics 12(7): e1006164.
PMID: 27379389

Sawala, A., Scarcia, M., Sutcliffe, C., Wilcockson, S. G. and Ashe, H. L. (2015) ‘Peak BMP Responses in the Drosophila Embryo Are Dependent on the Activation of Integrin Signaling’, Cell reports 12(10): 1584-1593.
PMID: 26321638

Newton, F. G., Harris, R. E., Sutcliffe, C. and Ashe, H. L. (2015) ‘Coordinate post-transcriptional repression of Dpp-dependent transcription factors attenuates signal range during development’, Development 142(19): 3362-3373.
PMID: 26293305

Winstanley, J., Sawala, A., Baldock, C. and Ashe, H. L. (2015) ‘Synthetic enzyme-substrate tethering obviates the Tolloid-ECM interaction during Drosophila BMP gradient formation’, Elife 4.
PMID: 25642644

Dunipace, L., Saunders, A., Ashe, H. L. and Stathopoulos, A. (2013) ‘Autoregulatory Feedback Controls Sequential Action of cis-Regulatory Modules at the brinker Locus’, Developmental Cell 26(5): 536-543.
PMID: 24044892

Saunders, A. and Ashe, H. L. (2013) ‘Taking a Pause to Reflect on Morphogenesis’, Cell 153(5): 941-943.
PMID: 23706731

Saunders, A., Core, L. J., Sutcliffe, C., Lis, J. T. and Ashe, H. L. (2013) ‘Extensive polymerase pausing during Drosophila axis patterning enables high-level and pliable transcription’, Genes & Development 27(10): 1146-1158.
PMID: 23699410

Sawala, A., Sutcliffe, C. and Ashe, H. L. (2012) ‘Multistep molecular mechanism for Bone morphogenetic protein extracellular transport in the Drosophila embryo’, Proceedings of the National Academy of Sciences of the United States of America 109(28): 11222-11227.
PMID: 22733779

Fritsch, C., Sawala, A., Harris, R., Maartens, A., Sutcliffe, C., Ashe, H. L. and Ray, R. P. (2012) ‘Different Requirements for Proteolytic Processing of Bone Morphogenetic Protein 5/6/7/8 Ligands in Drosophila melanogaster‘, Journal of Biological Chemistry 287(8): 5942-5953.
PMID: 22199351

Yarunin, A., Harris, R. E., Ashe, M. P. and Ashe, H. L. (2011) ‘Patterning of the Drosophila oocyte by a sequential translation repression program involving the d4EHP and Belle translational repressors’, RNA Biology 8(5): 904-912.
PMID: 21788736

Harris, R. E. and Ashe, H. L. (2011) ‘Cease and desist: modulating short-range Dpp signalling in the stem-cell niche’, EMBO Reports 12(6): 519-526.
PMID: 21546910

Harris, R. E., Pargett, M., Sutcliffe, C., Umulis, D. and Ashe, H. L. (2011) ‘Brat Promotes Stem Cell Differentiation via Control of a Bistable Switch that Restricts BMP Signaling’, Developmental Cell 20(1): 72-83.
PMID: 21238926

Ashe, H. L. (2008) ‘Type IV Collagens and Dpp Positive and negative regulators of signaling’, Fly 2(6): 313-315.
PMID: 19077532

Miles, W. O., Jaffray, E., Campbell, S. G., Takeda, S., Bayston, L. J., Basu, S. P., Li, M. F., Raftery, L. A., Ashe, M. P., Hay, R. T. and Ashe, H. L. (2008) ‘Medea SUMOylation restricts the signaling range of the Dpp morphogen in the Drosophila embryo’, Genes & Development 22(18): 2578-2590.
PMID: 18794353

Wang, X. M., Harris, R. E., Bayston, L. J. and Ashe, H. L. (2008) ‘Type IV Collagens regulate BMP signalling in Drosophila‘, Nature 455(7209): 72-U49.
PMID: 18701888

Ashe, H. L. and Briscoe, J. (2006) ‘The interpretation of morphogen gradients’, Development 133(3): 385-394.
PMID: 16410409

Ashe, H. L. (2005) ‘BMP signalling: Synergy and feedback create a step gradient’, Current Biology 15(10): R375-R377.
PMID: 15916937

Wharton, S. J., Basu, S. P. and Ashe, H. L. (2004) ‘Smad affinity can direct distinct readouts of the embryonic extracellular Dpp gradient in Drosophila‘, Current Biology 14(17): 1550-1558.
PMID: 15341741

Ashe, H. L. (2002) ‘BMP signalling: Visualisation of the Sog protein gradient’, Current Biology 12(8): R273-R275.
PMID: 11967165